Projects

These are projects already funded by STIRF

 

Increased vascular permeability: a possible mechanism for the increased cardiovascular risk seen in HIV patients treated with abacavir?

Project number: 008 (part two)

Background: An increased incidence of cardiovascular events (heart attacks and strokes) in the first six months of starting antiviral regimens containing abacavir has been reported in some studies but not others.

Aims: This project is the continuation of an investigation into the effect of long-term anti-HIV treatment on various aspects of metabolism (see Project 008 below).  In this part of the study the authors  examined the effect of antiviral treatment on genes in subcutaneous fat.

Methods: Subcutaneous fat biopsies were taken before, at 6- and 18-24-months after treatment with three commonly used antiretroviral regimens:

Zidovidine (AZT, lamivudine (3TC) and efavirenz
Tenofavir (TDF), emtricitabine (FTC) and efavirenz
Abacavir (ABC), lamivudine (3TC) and efavirenz

and from HIV negative controls.

Groups were matched for age, ethnicity, weight, body-fat distribution, biochemical profile, and pre-treatment CD4 count. Microarray data was generated on the extracted RNA.

Results: The study showed major effects of  abacavir-containing treatment regimens on genes controlling vascular permeability. The potential increased vascular ‘leakiness’, particularly marked marked in the first 6 months of starting treatment may provide possible mechanisms for the vascular plaques leading to heart attacks and strokes.

Dissemination: The study has been published in Plos One 2015 and would need confirmation by more detailed studies.

 

Why don’t the Somali Refugee Community Access Sexual Health Services.

Project number: 027

Background: In the United Kingdom there is an established and growing refugee population from Somalia. Despite this Somalis have remained absent from much of the official statistics largely because ‘Somali’ is rarely recognised as a distinct ethnic category. Consequently, when decisions are being made in relation to addressing the needs of recently arrived refugees the needs of those who originate from Somalia may be neglected. Little is known about the sexual health needs of this particular community but in terms of their broader health issues, Somalis are known to have a high level of need but low uptake of health care services (Carswell et al. 2011).

Aims: Through the use of focus groups and individual interviews this study sought to explore the sexual health needs of the local Somali community by ascertaining from their perspective, what they know about sexual health services, the challenges that may prevent them taking up these services and how services could be adapted to best meet their needs.

Results: Lack of knowledge of services, lack of knowledge about sexual health, issues pertaining to accessing services, shame, stigma and taboo, the influence of gender, religious and cultural norms, the perceptions of young people, the language barrier lack of cultural awareness and sensitivity demonstrated by healthcare professionals.

Recommendations:

  • Urgent attention be given to raising awareness, amongst the Somali community, about local sexual health services and how they can be accessed.
  • Information about sexual health services be made available in a format that is accessible to the Somali refugee community, for example, via a CD, DVD or USB stick.
  • That public health professionals and health care practitioners make clear the concept of preventative screening, making explicit the value of screening and early diagnosis for infectious diseases including TB and HIV/AIDS.
  • Health practitioners and public health professionals exercise extreme sensitivity when discussing issues related to sexual health and well-being. Every effort must be made to gain the trust of the individual so that they are able to discuss their fears or concerns.
  • Public health professionals and health care practitioners receive appropriate training and education so that they are equipped with the necessary cultural understanding and skills when working with the Somali community.
  • That a proactive approach be taken to helping Somali refugees to attend a programme of induction that includes language classes.
  • That a register of trained interpreters, who understand medical terminology and who are trained to work with those who may have experienced rape and torture should be available for public health professionals and health practitioners. Telephone interpretation should also be made available during consultations.

Dissemination: The results of the study have been presented in international meetings and published in reputable international journals.

It is hoped that results from this research can be used to inform service provision by identifying barriers to access and any opportunities that may help to overcome them.

 

Designing a research project for understanding the role of stigma and shame in STI and HIV testing.

Project number: 026

Later diagnosis of STI and HIV bring significant costs. Increasing take-up of regular testing and disclosure of one’s status to future sexual partners will bring benefit to individuals and also reduce overall rate of new infection.

The authors believe that shame is a significant factor in preventing access to sexual health facilities as well as partner notification. The study aims to find and validate the methodology or investigating the role of shame in STI and HIV testing. This is a preliminary feasibility study before undertaking a more in depth study.

 

Antimicrobial resistance in Chlamydia trachomatis: is it a reality?

Project number: 022

Background: Infections of the genital tract caused by Chlamydia trachomatis is the most common bacterial sexually transmitted infection (STI) in the UK. The burden of infection is seen in young people, with 147,594 infections diagnosed in 15 to 24 year olds in England in 2011. Chlamydia frequently does not cause any symptoms. Untreated infection can spread to cause pelvic inflammatory clisease (PID) in women with serious complications such as infertility and ectopic pregnancy.

The need to identify infected indivicluals has been recognised by the government who have funded the National Chlamydia Screening Programme (NCSP). This has resulted in large numbers of individuals receiving treatment in addition to those already attending specialised sexual health clinics. Resistance to antibiotics is currently rarely reported but there is concern that extensive use may select for resistant strains. Published and anecdotal evidence exists of patients who have persistent infection that is resistant to therapy.

Aims: This project aims to determine if there is evidence that Chlamydia resistance to antibiotics exists.

Methods: Specimens are collected and isolates from individuals that have failed antibiotic treatment are tested for resistance. A control group of individuals will be asked to provide an additional sample for Chlamydia culture prior to treatment to see prevalence of Chlamydia resistance in community

Project STIRF-022 has been completed 

 

Development of a weighting scale to evaluate the relative importance of items in a validated HIV patient satisfaction questionnaire. 

 Project number: 020

This project is a continuation of STIRF 012. It applied the validated questionnaire by determining the relative importance of the questions to patients. To achieve the study aims to develop a rating score and to obtain a weighted scale as to the importance of these questions to patients. This will allow service providers to tailor services to patients needs.

Project STIRF 020 has been completed 

 

Testing for chlamydia and gonorrhoea: persistence of a positive test result after successful treatment.

Project number: 015

Aims: Chlamydia and gonorrhoea are the most common treatable bacterial sexually transmitted infections diagnosed within the United Kingdom. Most sexual health clinics now use Nucleic Acid Amplification based Tests (NAATs), which test for DNA sequences particular to the organism in question, to diagnose these infections.

These tests are highly sensitive and specific but can remain positive for a while after the infection is successfully treated with antibiotics. This is because NAAT tests, unlike bacterial culture, do not distinguish between live and dead organisms. If individuals are retested too soon after completing treatment, their test result will still be positive from the initial treated infection. We do not know for how long this will be the case, making interpretation of repeat tests difficult. This is important in clinical practice, since there are a number of clinical scenarios (e.g. pregnancy, gonorrhoea infection) where a test of cure is essential.

Methods: The proposed project involves following up a cohort of individuals known to have chlamydia or gonorrhoea at a particular site for a period of six weeks. These individuals will be treated as per national guidelines and then retested every week (using NAAT tests) for the initial infection until this is no longer detected. Non-compliance and possible re-infection will be ruled out through structured questionnaires during the follow up period.

 

Development of a patient derived measure of satisfaction with HIV services

Project number: 012

Aims: The aim of this study is to develop, validate and test a local questionnaire to objectively measure patient satisfaction with HIV services. We had previously performed a systematic review of the instruments measuring patient satisfaction with HIV services and did not find a ‘gold standard’ measure by which services could be evaluated.  The study has been divided into two work streams.

Work stream 1: Development of a local Survey Tool using focus groups and in depth interviews.

Work stream 2: Qualitative assessment of patient barriers to the completion of satisfaction questionnaires.

Results: The focus groups identified key questions that need to be covered in the more in-depth questionnaire. In the next phase an assessment will be been made of the patients’ barrier to completion of the questionnaire.

Future use: The results will allow the construction of a questionnaire that will address the concerns of patients attending settings delivering HIV care. The questionnaire will then be used and evaluated in a field study.

Publication: The results have been published in International Journal of STD AIDS July 2011 22:366—367; doi:10.1258/ijsa.2011.010319

Collaboration between the University of Yale (USA) and Kazan (Russian Republic of Tataristan) and Birmingham to conduct courses in the antiviral treatment of HIV

Project number: 010

Aims: The epidemic of HIV in Russia and Eastern Europe has been explosive and threatens the health of the European continent as a whole. The collaboration was set up to organise regular courses to teach those directly involved with the care of patients infected with HIV how to use anti-HIV drugs correctly and effectively. The effective use of these drugs not only improves the immediate health and survival of the patient, but also dramatically reduces their ability to infect others. Treatment has both a clinical and an epidemiological role in reducing the spread of HIV.

Results: Seven annual two-week intensive courses were organised to train and update health care workers. The project covered the cities of Kazan and Chelney in Tataristan and Ufa in neighbouring Bashkurdistan province (once only). STIRF part funded the travel costs of one participant from the University of Birmingham (MS) on two occasions.

Future prospects: The conferences are now an annual event covering the training of hundreds of healthcare workers in all aspects of HIV care. The result has been a dramatic increase in the number of patients taking treatment in Tataristan which is now one of the leading centres of HIV management in the Russian Federation. The long term effects of this programme on the spread of HIV within Russia and beyond needs further study.

The effect of different antiviral treatments of HIV on genes controlling fat and energy metabolism in subcutaneous fat

Project number: 008 (part one)

Aims: With the discovery of treatment of HIV, the infection is no longer a death sentence and patients could look forward to a normal life span. However many of the treatments that control the virus were found to affect the metabolism of fat and sugars in the body so that there was an increased incidence of heart disease, diabetes and cancer – diseases associated with aging – at a  much younger age.

Results: The study found that there was a profound difference between antiviral regimens that are currently in use in the way they affect key genes controlling fat and energy metabolism. However, all the regimens appeared to impair efficient use of oxygen as fuel  and create conditions that are associated with leakage of the highly toxic reactive oxygen species (ROS). Since ROS is known to speed up the aging process and also the appearance of cancer, these results are very important and will stimulate further research on finding treatment regimens that are less likely to produce ROS.

Dissemination: The results of the study have been presented in international meetings and published in reputable international journals.

Future use: Since ROS is known to speed up the aging process and also the appearance of cancer these results are very important and will stimulate further research to find treatment regimens that are less likely to produce ROS.

 

Is filter paper suitable for transporting samples of Chlamydia trachomatis?

Project number: 006

Aim: The best way to obtain intimate samples is for patients to take them in the privacy of their homes and send them through the post. Since posting liquids, such as urine, is fraught with technical difficulties, using samples soaked into filter paper and allowed to dry would be ideal.

Results: The study showed that it is technically possible to extract the samples from the dried filter paper without much loss of accuracy.

Future use: The work now needs to be tested in the field.